All-fiber all-optical quantitative polymerase chain reaction (qPCR)

All-solid all-chalcogenide microstructured optical fiber.

The realization of an all-solid microstructured optical fiber based on chalcogenide glasses was achieved. The fiber presents As(2)S(3) inclusions selected as low refractive index material (n = 2.4) embedded in a As(38)Se(62) glass matrix (n = 2.8). The single mode regime of the fiber was demonstrated both theoretically by multipole method calculations and experimentally by near field measuremen...

Quantitative Real-time Polymerase Chain Reaction (qRT-PCR). Fatemeh Mehrpooyan*

All-fiber Optical Parametric Oscillator

All-fiber optical parametric oscillator (FOPO) has received considerable attention throughout these years. In this paper, we review the basic theory—four wave mixing (FWM) in the optical fibers, which leads to the parametric amplification. Phase matching condition is then discussed, with emphasis on the phase matching near the zero dispersion wavelength. In the end, recent developments on FOPO ...

qpcR: an R package for sigmoidal model selection in quantitative real-time polymerase chain reaction analysis

UNLABELLED The qpcR library is an add-on to the free R statistical environment performing sigmoidal model selection in real-time quantitative polymerase chain reaction (PCR) data analysis. Additionally, the package implements the most commonly used algorithms for real-time PCR data analysis and is capable of extensive statistical comparison for the selection and evaluation of the different mode...

Quantitative Real-Time Polymerase Chain Reaction (qPCR) of Filamentous Fungi in Carpet

We developed a protocol for the rapid identification and quantitation of fungi by quantitative real-time polymerase chain reaction (qPCR) in carpet. The fungi used in this study are field isolates of Alternaria alternata, Aspergillus versicolor, Cladosporium cladosporoides, and Stachybotrys chartarum. The modified spore extraction method provided superior quality, high-molecular-weight genomic ...